Pdgfrb-creer tdtomato

Author: edhc

August undefined, 2024

Splet01. feb. 2024 · The protein encoded by the PDGFRB gene is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family [27]. PDGFRB plays an … Splet12. apr. 2024 · To elucidate the heterogeneity of fibroblasts derived from tdTomato + cells and the potential role of fibroblasts in this HLI model, we performed scRNA-seq analyses of tdTomato + cells that were sorted by fluorescence-activated cell sorting (FACS) from a single-cell suspension of muscle tissues from tamoxifen-treated Cd34-CreER T2;R26 …

Contribution of PDGFRα-positive cells in maintenance and injury ...

Splet01. avg. 2011 · The inducible PDGFRβ-P2A-CreER T2 model has been validated with coimmunostaining and time course experiments to test pericyte expression and has high … Splet07. mar. 2024 · To confirm efficiency of the intraperitoneal tamoxifen administration, a Pdgfrb -CreER mouse was also induced via local administration of 10 μL into the footpad to drain directly into the PLVs for 5 consecutive days. Evaluation of tdT + cells after tamoxifen induction was performed at least 7 days after the final injection. the man at the well

030201 - PDGFRβ-P2A-CreER[T2] Strain Details - The …

Splet08. okt. 2008 · When Pdgfra-creER T2 /Rosa26-YFP mice were analyzed 5–28 d post-tamoxifen, the number of YFP + cells increased in both corpus callosum and cerebral cortex and an increasing fraction of them were ... Splet21. apr. 2024 · We then treated Pdgfra-CreER; R26 tdTomato mice with tamoxifen and harvested the carotid artery and ascending aorta on day 10 to examine the initial labeling … Spletmouse line: Pdgfrb-CreER;tdTomato treatment: TAC surgery cell type: tdTomato-positive heart cells Stage: Pressure overload induced cardiac remodeling Treatment protocol After a washout period of 21 days, transverse aortic constriction (TAC) was performed to induce cardiac hypertrophy or Sham surgery as control procedure. Growth protocol the man at the window 2

Dual lineage tracing identifies intermediate mesenchymal stage …

Pdgfrb-creer tdtomato

029684 - B6.Cg-Tg(Pdgfrb-cre/ERT2)6096Rha/J

Splet03. jun. 2024 · PDGFRa is a marker used for perivascular adventitial cells or fibroblasts, and by examining heart and adipose tissue sections collected from Pdgfra-DreER;R26-rox-Stop-rox (RSR)-tdT, we found that PDGFRa + cells were specifically and efficiently labeled ( Figure 2 A; Figures S2 A–S2C). Splet01. jul. 2005 · KIAA1509 is a novel PDGFRB fusion partner in imatinib-responsive myeloproliferative disease associated with a t(5;14)(q33;q32). Levine RL et al: 11588050: …

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SpletThe NG2-CreER™-tdTomato and the PDGFRb-P2A-CreER T2-tdTomato mice were treated with tamoxifen at three defining time points of retinal vascular development: post-natal … Splet14. apr. 2024 · Pdgfrb_Sham: Sample type: SRA : Source name: heart cells: Organism: Mus musculus: Characteristics: mouse line: Pdgfrb-CreER;tdTomato treatment: Sham surgery …

Splet本发明提供一种Cx3cr1CreER‑Ai27基因型双转鼠以及一种新的小胶质细胞激活方法，通过Cx3cr1CreER小鼠与Ai27基因小鼠进行杂交获得Cx3cr1CreER‑Ai27基因型双转鼠，在腹腔注射Tamoxifen后于目标脑区植入光纤，使用蓝光(450‑490nm)刺激，即可特异性激活光纤下方区域小胶质细胞。 Splet01. jul. 2024 · PDGFRβ-P2A-CreER T2 mice allow for tamoxifen-inducible expression of Cre-recombinase under the control of the platelet derived growth factor receptor-β (PDGFRβ) promoter, which is expressed in...

Splet07. mar. 2024 · The PDGFRα-CreER transgenic mouse was first developed to study oligodendrocyte precursor cells in the forebrain and spinal cord of adult mice. 27 The … Splet030201 B6.Cg- Pdgfrb tm1.1(cre/ERT2)Csln /J PDGFRβ-P2A-CreER T2 mice express tamoxifen-inducible Cre recombinase under the direction of the murine platelet derived growth factor receptor, beta polypeptide ( Pdgfrb ) promoter. They are useful for applications requiring tamoxifen-induced deletion of floxed sequences in vascular …

Splet图2 经他莫昔芬诱导后，tdtomato表达在Prox1-CreER; Rosa26-tdTomato小鼠的海马体齿状回处。图3 经他莫昔芬诱导后，tdtomato还会在垂体、视网膜、心脏、胸腺、胃、肺、颌下腺、肌肉、卵巢、脾脏、胰腺、肾等器官或组织中出现信号。(想要获取更多信息需联系技术 …

Splet25. nov. 2024 · dTomato来源于一种被命名为dimer2的中间体，这种中间体是在四聚体DsRed蛋白崩解过程中产生的。这种变体包含GFP的N末端和C末端的前后各七个氨基酸残基，这样可以提高与融合蛋白的兼容性并降低 … the man at the wheel paintingSplet30. maj 2024 · Fgd5-CreER; tdTomato mice were injected with tamoxifen for 5 days, then with 5-FU at 2 weeks after the first tamoxifen injection. Peripheral blood (PB) was analyzed at weeks 3 and 4 (n = 3-4). (F) Peripheral blood analysis for tdTomato + cells in the indicated population revealed increased tdTomato + cells after 5-FU treatment. All data ... the man at the window bookSpletThe PDGFRB gene is located on human chromosome 5 at position q32 (designated as 5q32) and contains 25 exons. The gene is flanked by the genes for granulocyte … tidy cat blue lidSplet21. apr. 2024 · We then treated Pdgfra-CreER; R26 tdTomato mice with tamoxifen and harvested the carotid artery and ascending aorta on day 10 to examine the initial labeling (Figs. 1 C–E). While labeled cells... tidy cat breeze cat refill padsSplet31. maj 2024 · E, immunostaining for tdTomato, CDH5, and PDGFRb on heart sections from adult Nfatc1–Dre;Sox9–CreER;Ai66. The arrows indicate tdTomato + PDGFRb + pericytes. The boxed regions in the left panel are magnified and split channels in the right panels. Scale bars, 100 μm. tidy cat boxSplet南模生物为该研究构建了多种基因修饰小鼠： R26-LSL-RSR-tdT-DTR, Tnnt2-Dre-Cre-tdTomato, Npr3-tTA以及Col1a2-RSR-CreER等。之前的研究主要用单同源重组酶介导的谱系示踪方法，通过心内膜的遗传工具小鼠和心外膜的遗传工具小鼠去研究心内膜衍生的成纤维细胞和心外膜衍生 ... the man at the window downloadSplet22. feb. 2024 · These results suggest that PDGFRβ signalling plays a critical role in regulating fibrous cap development, but fail to provide direct insight into the role of PDGFRβ signalling in SMCs and its role... themanatur.eu