FlipGFP is a novel fluorogenic reporter that has a large dynamic range and high brightness. This reporter achieves approximately 100-fold increase in fluorescence after cleavage by executioner caspases. Failure for cells to die or cells dying at the wrong time and/or place can lead to an array of diseases and … See more GFP contains 11 β-strands and one central ɑ-helix that can be split into 3 parts: (1) β-strands 1-9 (β1-9) and the one ɑ-helix, (2) β-strand 10 (β10), and (3) β-strand 11 (β11). Each part contains components and … See more To add to the fluorogenic reporter toolkit, the Shu Lab also designed a red fluorogenic protease reporter based on superfolder Cherry … See more The Shu Lab successfully used FlipGFP to study apoptosis in HeLa cells, zebrafish embryos, and Drosophila. They treated HeLa cells expressing FlipGFP with staurosporine, which … See more WebSignificantly, we developed a cell-based FlipGFP assay that can be applied to predict the cellular antiviral activity of PLpro inhibitors in the BSL-2 setting. X-ray crystal structure of PLpro in complex with GRL0617 showed that binding of GRL0617 to SARS-CoV-2 induced a conformational change in the BL2 loop to a more closed conformation.
Development of a fluorescence based, high-throughput SARS …
WebThis assay is performed in human cell culture and does not require 86 biosafety level 3 (BSL3) containment. Our reporter is based on FlipGFP, which only fluoresces 87 after … WebSep 2, 2024 · A FlipGFP reporter assay was performed to screen for potential inhibition of 3CLpro by the identified drugs at a single concentration (10µM). Shown are the drugs that showed a statistically ... portable marine gas tank fittings
Addgene: COVID-19 Resources
WebNov 4, 2024 · FlipGFP PLpro assay with the positive control GRL0617 and the negative control GC-376. Dose−response curves of the ratio of GFP/mCherry fluorescence with SARS-CoV-2 PL pro inhibitors were plotted at right side column; mCherry signal alone was used to normalize protein WebPCDNA3-FlipGFP (Casp3 cleavage seq) T2A mCherry was a gift from Xiaokun Shu (Addgene plasmid # 124428 ; http://n2t.net/addgene:124428 ; RRID:Addgene_124428) … irs apply for efin