Coupled analysis of transcriptome and bcr
WebOct 15, 2014 · BCR stimulation actually downregulated ICOSL on both GC and non-GC B cells. ... nodes 120 h post-immunization and subjected to transcriptome analysis by RNA ... presentation coupled with ICOSL ... WebComparative transcriptome analysis of Brachypodium roots with rice roots during NLS formation showed that there are overlapping gene expression patterns between these …
Coupled analysis of transcriptome and bcr
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WebAbstract. Single-cell transcriptome sequencing (scRNA-seq) is an unparalleled technology in the field of transcriptomics, which can study cells in a comprehensive and unbiased manner, thus characterizing complex biological processes at the cellular level. At the same time, scRNA-seq can produce a large amount of experimental data; therefore ... WebJul 20, 2024 · Here, we used single-cell transcriptome and TCR sequencing to systematically characterize the composition, function and lineage relationship of T …
WebCoupled analysis of somatic hypermutation in immunoglobulin heavy chain (Igh) variable gene regions showed that GC B cells acquiring higher-affinity mutations … WebAug 24, 2024 · B cell responses are a critical component of anti-viral immunity. However, a comprehensive picture of antigen-specific B cell responses, differentiation, clonal proliferation and dynamics in different organs after infection is lacking. Here, we combined single-cell RNA sequencing with single-cell B cell receptor (BCR) characterization of …
WebTranscriptome analysis technologies are important systems-biology methods for the investigation and optimization of mammalian cell cultures concerning with regard to growth rates and productivity. For the production of recombinant proteins, knowledge of the expression conditions of the influencing genes is a major issue in the improvement of ... WebBD Rhapsody™ TCR/BCR Profiling Assays for Human and Mouse; BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit ... as CD197, on the cell surface. CCR7 (previously known as BLR2, EBI1 and CMKBR7) is a seven-transmembrane, G-protein-coupled receptor specific for two CC chemokines: CCL19 (also known as MIP-3β, …
WebOct 4, 2024 · In the first step, only cells with both transcriptome and TCR/BCR information were retained for downstream analysis. Next, only cells with paired light/heavy chains …
WebJun 18, 2024 · Systemic lupus erythematosus (SLE), which was recognized as a defined clinical entity more than 100 years ago, is an archetype for systemic autoimmune diseases. The 10-year survival of SLE patients has shown dramatic improvement during the last half-century. However, SLE patients receiving long-term prednisone therapy are at high risk … guy who ran down a paradeWebMay 24, 2024 · Antigen-activated B cells diversify variable regions of B cell antigen receptors by somatic hypermutation in germinal centers (GCs). The positive selection of GC B cells that acquire high-affinity mutations enables antibody affinity maturation. In spite of considerable progress, the genomic states underlying this process remain to be … boy george attack on manWebSep 11, 2024 · The basis of expressome assembly and its consequences for transcription and translation are poorly understood. Here, we present a series of structures … boy george and jon moss relationshipWebApr 11, 2024 · The user can then select the most significantly correlated parameter-module pair and proceed with pathway analysis or network analysis of the genes in the module It was seen that the most significantly correlated module for the BCR-ABL1 patients was “midnight blue” (p-value = 0.002, Pearson correlation value, r = 0.85) with 91 genes that ... guy who ran over people in wisconsinWebJun 22, 2024 · Combining single-cell RNA and BCR sequencing, Mathew et al. report that, after influenza infection, memory B cells (Bmems) are stochastically selected from both low- and high-affinity germinal center (GC) precursors. They reveal that lung Bmems acquire tissue-residency signatures and continuously differentiate from the spleen and … guy who ran people over at paradeWebJul 1, 2024 · Coupled analysis of transcriptional states and Igh variable sequences revealed that OXPHOS metabolism is enhanced in GC B cells undergoing positive … guy who sang never gonna give you upWebMay 24, 2024 · The coupled analysis of transcriptional states and Igh variable sequences revealed that the OXPHOS module is enhanced in GC B cells undergoing positive selection in their responses to simple as... Extended Data Fig. 2 Topic Modeling, Pseudotime and 2-Nbdg Uptake … Extended Data Fig. 5 Seahorse, Flow Cytometry and ELISA Analysis of Cox10 … Extended Data Fig. 6 Flow Cytometry Analysis of iCG B Cells and T Fh Cells - … boy george band aid