Clonexpress multis one step cloning
Web陈合凤，张 帅，王喜军，王金良，温志远，葛金英，陈伟业*，步志高,2* （1.中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室，黑龙江 哈尔滨 150069；2.江苏高校动物重要疫病与人兽共患病防控协同创新中心，江苏 扬州 225009） WebClonExpress MultiS One Step Cloning Kit is a new version cloning kit based on ClonExpress One Step Cloning technology. Exnase MultiS and reaction buffer in this kit are especially optimized for the assembly of multiple fragments. With the help of this kit, up to five fragments can be orderly assembled in a single reaction. In addition, Exnase ...
Clonexpress multis one step cloning
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WebJun 17, 2024 · Application Note: ClonExpress Ultra One Step Cloning Kit 17 June 2024 In this application note, Vazyme describes its ClonExpress Ultra One Step Cloning Kit (Vazyme #C115), designed to meet the growing demand for … WebApr 6, 2024 · For pBS-EMR, the ef-1α B promoter and rap-1 terminator were amplified from the B. duncani genome, the mCherry fragment was amplified from the plasmid of pBS-PAC-mCherry, and these fragments were cloned separately into the pBluescript (pBS) backbone plasmid using the ClonExpress MultiS One Step Cloning Kit (Vazyme, China).
WebJul 8, 2024 · Briefly, upstream and downstream sequences of target gene were amplified from mutants and purified PCR products were cloned into the pMo130-Hyg R vector using ClonExpress® MultiS One Step Cloning Kit (Vazyme biotech Co, Nanjing, China) via BamHI and XbaІ restriction sites. The primers used are listed in Supplementary Table 1. WebOct 16, 2024 · To generate pCAMBIA1300-3xFLAG-NbAGO4-1, pCAMBIA1300 was digested with XbaI and XmaI, and then the 3xFLAG and NbAGO4-1 coding sequences were amplified by PCR and Infusion-cloned into pCAMBIA1300 with ClonExpress MultiS One Step Cloning Kit (Vazyme). pDONR207-Fibrillarin (Kim et al., 2007b) was used to …
WebCloning/Mutagenesis; Nucleic Acid Electrophoresis; Reverse Transcription; Real-Time PCR (qPCR) Genome Editing; Cell Biology/Protein Research; Reagents for NGS Library Preparation. Automated Liquid Handling Workstation; DNA Sequencing; RNA Sequencing; Single Cell-seq Series; Epigenetics Series; Beads Series; Quantification Series; Capture … WebClonExpress® technology is a simple, fast and efficient DNA seamless cloning technology, which can clone the insert into any position of any vector. Use any method to linearize …
Web· · Ace-Hi One Step qPCR-PCR SYBR Green Kit Ace-Hi One Step qPCR-PCR Probe Kit · · ClonExpress II One Step Cloning Kit ClonExpress MultiS One Step Cloning Kit 克 隆 到 克 目 隆 的 到 载 体 载 体 T · · · · · 重组后的质粒DNA ·
WebProduct Introduction. Mut Express® II Fast Mutagenesis Kit V2 is a site-directed mutagenesis system based on ClonExpress® rapid cloning technology. Using this kit, the target plasmid amplification product is digested with DpnI, ClonExpress® recombination and circularized, and then directly transformed to complete the site-directed mutation. ferdinand a bika teljes film magyarul videaWebIt can efficiently clone 50 bp - 10 kb fragments and carry out one step multi-fragment seamless cloning. Enzyme digestion is unnecessary. It only needs to introduce the … ferdinánd a bikaWebThe ClonExpressTM MultiS (One Step Cloning) Kit provides a convenience, fast, high-throughput cloning of DNA fragment and generation of precise, directional constructs … hp 240 g8 data sheetWebThe cloning products can then be directly transformed to competent cells with a positive rate > 95%. ClonExpress MultiS One Step Cloning Kit is a new version cloning kit based on ClonExpress One Step Cloning technology. Exnase MultiS and reaction buffer in this kit are especially optimized for the assembly of multiple fragments. ferdinand 2 teljes film magyarul videaWebMar 2, 2024 · All building block plasmids and backbone plasmids were constructed using the ClonExpress ® MultiS One Step Cloning Kit (Vazyme Biotech Co., Ltd., Nanjing, China). All plasmids used in this study and the sequences of building blocks and backbone plasmids are listed in Supplementary Tables S1, S2. hp - 240 g8 - dark ash silverWebIt can efficiently clone 50 bp - 10 kb fragments and carry out one step multi-fragment seamless cloning. Enzyme digestion is unnecessary. It only needs to introduce the homologous sequence at the end of the vector at the 5' end of the primer. The linearized clone vector and PCR products can be cloned directly without purification. hp 240 g8 i7 spesifikasiWebThe corresponding primers used for gene cloning are listed in Supplementary Table 1. The PCR products were purified and cloned into the EcoRI and ... digested pTAex3 using the ClonExpress® MultiS One Step Cloning Kit. For constructing plasmids harboring two or three genes, DNA fragments, including the amylase promoter and terminator, were ... ferdinand a bika teljes magyarul teljes film